Question
An enzyme with a Km of 1x10 M was assayed using an initial substrate concentration of 3x10 M. After 2 min, 5 percent of
An enzyme with a Km of 1x10 M was assayed using an initial substrate concentration of 3x10 M. After 2 min, 5 percent of the substrate was converted. How much substrate will be converted after 10, 30, and 60 min? (Note: Solve using the integrated form of the Michaelis-Menton equation.) Tyrosinase has a Km of 9.2 g/l. The reactant is p-aminophenol. Acetone which acts as an inhibitor, is present in the reaction mixture at a level of 1 g/l. The following data were obtained in the presence of acetone. Is acetone competitive or uncompetitive? Explain? Calculate K. 0.909 0.658 0.493 0,400 0.333 0.289 0.227 [R] (g/1) 20 10 6.67 5 4 3.33 2.5
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