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An outbreak of Shigella has been traced to food contaminated by ill food handlers. Shigellosis is an acute gastrointestinal infection caused by bacteria belonging to

An outbreak of Shigella has been traced to food contaminated by ill food handlers. Shigellosis is an acute gastrointestinal infection caused by bacteria belonging to the genus Shigella. You are expected to detect the two main Shiga toxins: STEC: STX 1 (800bp) and STX 2 (1200bp).What will be your best samples to be used for this analysis; and what will be your approach to detect these markers? There is an emerging global trend of S. sonnei replacing S. flexneri in Greater Accra. Even though you did not design primers to target this new strain; your gel bands showed a third Shiga toxin of molecular size of 700 bp. List 3 possibilities that account for the detection of this third product. . What will be your next line of action before disclosing the results to the medical team?

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