analytical chemistry

Question 7: Fluorescence measurements

a) Fluorescence measurements can give detection limits that are more than 1000 times lower than those obtained by absorbance measurements on the same substances. Describe two different reasons for this. Motivate!

b) In quantitative fluorescence measurements, it can be an advantage to use a laser as a radiation source, while this is rarely the case in absorbance measurements. Explain why.Motivate!

c) Quenching can occur during fluorescence measurements. Describe this phenomenon and what effects it can have. Motivate!