Answer the following questions using the crystal structure of 4DKL from the Protein Data Bank website. (https://www.rcsb.org/structure/4dkl)

1		GSHSLCPQTG SPSMVTAITI MALYSIVCVV GLFGNFLVMY VIVRYTKMKT
		HHHHHH HHHHHHHHHH HHHHHHHHHH HHHH STT S
51		ATNIYIFNLA LADALATSTL PFQSVNYLMG TWPFGNILCK IVISIDYYNM
		HHHHHHHHHH HHHHHHHHHH HHHHHHHHHS S S SHHHH HHHHHHHHHH
101		FTSIFTLCTM SVDRYIAVCH PVKALDFRTP RNAKIVNVCN WILSSAIGLP
		HHHHHHHHHH HHHHHHHHH HHHHHHH H HHHHHHHHHH HHHHHHHHHH
151		VMFMATTKYR QGSIDCTLTF SHPTWYWENL LKICVFIFAF IMPVLIITVC
		HHHSB EEE TTEEE B SSSHHHHHHH HHHHHHHHTT HHHHHHHHHH
201		YGLMILRLKS VRNIFEMLRI DEGLRLKIYK NTEGYYTIGI GHLLTKSPSL
		HHHHHHH SHHHHHHH HH EEEEEE TTS EEEET TEEEESSS S
251		NAAKSELDKA IGRNTNGVIT KDEAEKLFNQ DVDAAVRGIL RNAKLKPVYD
		HHHHHHHHHH HSS TTB HHHHHHHHHH HHHHHHHHHH T TTHHHHHH
301		SLDAVRRAAL INMVFQMGET GVAGFTNSLR MLQQKRWDEA AVNLAKSRWY
		TS HHHHHHH HHHHHHH HH HHHT HHHHH HHHTT HHHH HHHHTTSHHH
351		NQTPNRAKRV ITTFRTGTWD AYEKDRNLRR ITRMVLVVVA VFIVCWTPIH
		HHSHHHHHHH HHHHHHSSST TTS HHHHH HHHHHHHHHH HHHHHHHHHH
401		IYVIIKALIT IPETTFQTVS WHFCIALGYT NSCLNPVLYA FLDENFKRCF
		HHHHHHHHS HHHHHH HHHHHHHHHH HHHHHHHHHH HH HHHHHHH
451		REFCIPTSST IEQP
		HHHH

Use the amino acid sequence from above to answer the following questions.

Use a protein BLAST search to identify potential homologs in the organisms indicated in the table below. Include the gene name and E score for the top result.

Organism	gene name	E score
Saccharomyces cerevisiae
Drosophila melanogastor
Caenorhabditis elegans

What does the E score mean?

Using the E score as a rationale, which organism(s) has a clear homolog?

Which organism(s) does not have a clear homolog?

Based on what we discussed in class for each organism (unicellular vs metazoan), use no more than a few short sentences to speculate why one organism might encode the protein and another may not. Your reasoning should include reference to the type of protein and how it works in general terms.