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answer the three questions please In anionic exchange chromatography. At the end eluent with low pH tacidic eg. H+ ion solution) pass through, that releases

answer the three questions please
In anionic exchange chromatography. At the end eluent with low pH tacidic eg. H+ ion solution) pass through, that releases the anionic or negatively charged protein Question 32 Given the following pKa's, the isoelectric point of Arginine is pK1(COOH)=2.17 pK2 (+NH3)=9.04 pK ( sidechain +NH3)=12.48 10.76 5.605 7.325 8.99 Given that pK1=2.35 and pK2=9.69 for alanine. What is its isoelectric point? 6.02 9.69 2.34 12.03

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