Assignment problem:

A biologist who acquired three fluorescence microscopy images of cells wants to make quantitative

measurements and know whether $($and to what extent$)$ the areas of the cells may differ from one image to

another. They say that the cells should be in the $25-175$ range in terms of area $($in pixels$).$

Download the three images and the sample FIJI and R macros. Edit the macros according to your own

decisions, computer architecture, etc. and make sure all lines of code are commented.

Image processing:

Segment the cells. Which automated thresholding algorithm did you use and why?

Did you use the same automated thresholding algorithm for each image? Explain.

Statistical analysis in R:

How many cells did you find for each image?

For each image, what is the mean cell area? What is the median cell area?

In a single chart, plot three histograms of cell areas, of three different colors, one for each image.

In a single chart, plot three boxplots of cells areas, of three different colors, one for each image.

For each image, are the cell areas normally distributed? Explain. What does this tell you about the

possibility of using the mean and standard deviation as representative estimators for the data?

Is there a statistically significant difference in cell area between each of the images? Explain.

Provide fiji scripts, threshold images, and the statistal analysis code with the explanations