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At 25C under physiological standard-state conditions (all substrates and products at 1.0 M except H+) the hydrolysis of asparagine to aspartate + NH4+ has a

At 25C under physiological standard-state conditions (all substrates and products at 1.0 M except H+) the hydrolysis of asparagine to aspartate + NH4+ has a G' of -13.6 kJ/mol. At the same temperature and standards state conditions the hydrolysis of ATP to AMP and PPi has a G' of -32 kJ/mol. From this information, calculate the G' for asparagine biosynthesis from aspartate via the overall reaction: Aspartate + ATP + NH4+ Asparagine + PPi + AMP

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