AT: L-aspartate +-ketoglutarate oxalacetate + L-glutamate DH: oxalacetate + NADHLmalate+NAD+ What change is being directly detected by the spectrophotometer? (8 pts.) incorrect The absorbance change due to the chemical reduction of NADH. incorrect The increase in absorbance due to the loss of NADH. Incorrect The decrease in absorbance due to the chemical reduction of oxalacetate. Incorrect The absorbance change due to a product of the MDH reaction. Correct: The absorbance change due to the change in concentration of a substrate of the GDH reaction. At what wavelength is this change being detected? (4 pts.) 340nm For every mole of L-aspartate converted, how many moles of MDH are consumed (enter a number, not a word)? (4 pts.) 0 Previous Tries What is the starting absorbance for the AAT assay as described in the Unit C background, subsection Specifc Assays/Assay for AAT activity, portion of the Course Pack ( Ppage 57-59) when measuring the endogenous activity (assume that the diluted fraction does not contribute to the absorbance at this waveiength)? (4 pts.) 0 Tries 2/5 Previous Tries. What would be the absorbance following the addition of a-ketoglutarate? (4 pts.) Tries 0/5 You dilute your fraction 300 -fold, have the chart recorder adjusted to 0,5 absorbance per 100 chart squares, and perform the Aat assay as desonbed in the coursepack. You observe no endogenous activity. Calculate a conversion facto, Cf, that would satisfy the following equation: chart squares /mincr=1m/mL of undiluted fraction. (8 pts.)