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(b) A colored substance E has an absorption maximum at 350nm. A solution containing 2mmol/L of a compound E had an absorbance of 0.56 using
(b) A colored substance E has an absorption maximum at 350nm. A solution containing 2mmol/L of a compound E had an absorbance of 0.56 using a 1cm cuvette in a UV-Vis spectrometer. The molecular weight of E is 200g/mol. (i) Calculate the molar absorptivity of E at 350nm; (ii) Will there be any change in any of the parameters amongst 'absorbance', 'molar absorptivity' and 'absorption maximum', if the test was performed using a cuvette of path length 5cm ? - Justify showing reasens/ calculations as appropriate
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