Bacillus subtilis produces amylase, an enzyme. A medium containing 10 g/L of starch is inoculated with Bacillus subtilis. The production profile in a batch system during exponential phase is reported in Table Q4. Time (h) 2 3 4 Table 04: Production profile Cell concentration Starch concentration Amylase concentration (g/L) (g/L) 0.04 10.00 (mgL) 357 0.12 0 17 564 0.35 9.02 662 1.13 7.58 695 3.66 1.30 1213 (a) The production of amylase can be conducted either in batch or a continuous system. Differentiate the batch culture svstem from a continuous culture system. (6 marks) (b) With the aid of an appropriate graph, determine the specific growth rate (H) and doubling time (a) of Bacillus subtilis. (7 marks) (c) Calculate the yield of cell and product obtained from the batch culture system. Identify the maximum amount of cells and products concentration one could expect if 20 g/L of starch is fed into the same system. (8 marks) (d) Calculate the amount of cells produced (in cells number) after 2.5 hours if a 1.5 m' reactor is inoculated with 0.05 g/L of cells. Approximately 1 g of Bacillus subtilis is equivalent to 1 1012 cells (4 marks) [Total : 25 marks]