BACTERIAL GROWTH IN CONTINUOUS CULTURE Problem Set, Due Monday, April 4. 2022, 11:59 pm Consider a continuous flow reactor operating at a dilution rate of 0.5 hr-1, with the following growth constants: Im = 1.0 hr1, Y = 0.5, Ks = 0.1 g/L. 1. Determine the concentration of cells at steady state for entering substrate concentrations of 1.0 g/L, 3.0 g/L, 7.0 g/L. 2. What is the steady state substrate concentration for each of the entering substrate concentrations? 3. Determine the cell concentration and substrate concentration if the dilution rate was changed to 0.8 hr, for the above entering substrate concentrations. 4. What is the critical dilution rate above which washout of cells would occur for each entering substrate concentration? 5. On one graph, plot the change in cell concentrations and the change in substrate concentrations vs. dilution rate for each of the above mentioned entering substrate concentrations. Note: the graph should (generally) resemble the ones we discussed in class. 6. For a given dilution rate, what controls cell concentration? 7. What controls substrate concentration? [For Q.6 & 7 consider what you control as the operator of the system]