Begin by preparing

10mL

of a

100mM

stock solution of

CuSO_(4)

(MW

{

(

:=159.609(g)/(m)ol)

}.\ Begin by creating

2mL

of the following dilutions of your

100mMCuSO4

stock solution using distilled water:

(1)/(5),(1)/(10),(1)/(25),(1)/(50),(1)/(75)

and

(1)/(100)

.\ Determine the A700 for each dilution and an unknown sample marked "U1". Make sure the cuvette is placed properly into the spectrophotometer and you wipe the sides with a Kimwipe. Start with a blank

(dH_(2)O)

and zero the spec. then move onto the lowest concentration sample and move up sequentially using the same cuvette (pour the contents back into its original container). Use a new cuvette for each unknown.\ Assuming the

CuSO_(4)

solution is a

20x

stock, prepare the following solutions (final volume

=2mL

):

0.5x,1x,2x,5x

and

10x

.\ Determine the A700 for each dilution and an unknown sample marked "U2".\ Prepare the following solution of

CuSO_(4)

solution using serial dilutions (use the original stock):

(1)/(5)

,

(1)/(25),(1)/(125),(1)/(625)

.\ Determine the A700 for each dilution and an unknown sample marked "

U3

".

W) Begin by preparing 10mL of a 100mM2 stock solution of CuSO4(MW=159.609g/mol ). 2) Begin by creating 2mL of the following dilutions of your 100mMCuSO4 stock solution using distilled water: 1/5,1/10,1/25,1/50,1/75 and 1/100. 3) Determine the A700 for each dilution and an unknown sample marked "U1". Make sure the cuvette is placed properly into the spectrophotometer and you wipe the sides with a Kimwipe. Start with a blank (dH2O) and zero the spec. then move onto the lowest concentration sample and move up sequentially using the same cuvette (pour the contents back into its original container). Use a new cuvette for each unknown. 4) Assuming the CuSO4 solution is a 20X stock, prepare the following solutions (final volume =2mL ): 0.5X,1X,2X,5X and 10X. 5) Determine the A700 for each dilution and an unknown sample marked "U2". 6) Prepare the following solution of CuSO4 solution using serial dilutions (use the original stock): 1/5, 1/25,1/125,1/625. 7) Determine the A700 for each dilution and an unknown sample marked "U3