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bio chem The hydrobysis of pyrophosphate to orthophosphate drives biosyathetic reactions such as DNA synthesis. In Ercherichia cod, a. pyrophosphatase catalyzes this bydrolytic reaction. The
bio chem
The hydrobysis of pyrophosphate to orthophosphate drives biosyathetic reactions such as DNA synthesis. In Ercherichia cod, a. pyrophosphatase catalyzes this bydrolytic reaction. The pyrophosphatase has a mass of 120kDa and consists of ux identical subunits. A unit of activity for this enzyme, U, is the amount of enzyme that hydrolyzes 10mol of pyroplosphate in 15 minutes. The purified enzyme has a Vman of 2800U per milligram of enayme. When [S]Kwi how many micromoles of substrate can 1 mg of eaxymo hydrolyze per second? If each er site, how many micromoles of active sites, or [E]T, are there in 1mg of enhyme? [E]T = ymonol +mg1 What is the tarnover namber, or Acat of the erryme? kcal= Step by Step Solution
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