bio chem

The hydrobysis of pyrophosphate to orthophosphate drives biosyathetic reactions such as DNA synthesis. In Ercherichia cod, a. pyrophosphatase catalyzes this bydrolytic reaction. The pyrophosphatase has a mass of 120kDa and consists of ux identical subunits. A unit of activity for this enzyme, U, is the amount of enzyme that hydrolyzes 10mol of pyroplosphate in 15 minutes. The purified enzyme has a Vman of 2800U per milligram of enayme. When [S]Kwi how many micromoles of substrate can 1 mg of eaxymo hydrolyze per second? If each er site, how many micromoles of active sites, or [E]T, are there in 1mg of enhyme? [E]T = ymonol +mg1 What is the tarnover namber, or Acat of the erryme? kcal=