BIOCHEMISTRY

Based on the structural properties of this protein, how resistant (or sensitive) would your protein be to heat denaturation and why? 150 words

Protein Ubiquitin-conjugating enzyme E2 2 Gene RADO Organism Saccharomyces cerevisiae (strain ATCC 204508 / S2880) (Baker's yeast) Molecule Chains Sequence Length Organism A, B, C 169 UBIQUITIN- CONJUGATING ENZYME RADO Saccharomyces cerevisiae Function: Catalyzes the covalent attachment of ubiquitin to other proteins. In association with the E3 enzyme BRE1 and LGE1, it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B to form H2BK123ub1. H2BK123ubi gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In association with the E3 enzyme RAD18, it catalyzes the monoubiquitination of POL30 'Lys-164', involved in postreplication repair of UV-damaged DNA. The RAD6/UBC2-RAD18 complex is also involved in prevention of spontaneous mutations caused by 7,8-dihydro-8-oxoguanine. In association with the E3 enzyme UBR1, is involved in N-end rule- dependent protein degradation. Also involved in sporulation