Biomass production is carried out in a continuous system. In the experiments, the optimum dilution ratio (Dopt) of the system in which the highest biomass yield was obtained was determined as 0.6 h-1. In addition, the maximum specific growth rate (max) value was found to be 0.9 h-1 and the substrate constant (KS) value was 100 mg/L in the steady state of the system. Calculate the substrate concentration (SI) entering the system and the optimum substrate output (Sopt) values at which the highest biomass yield is obtained, using the equations below.