Bits have no inherent meaning. Given the bit pattern:

1010 1100 1011 0010 0100 1001 0010 0100

What does it represent respectively, assuming that it is a twos complement integer?

I keep getting a negative number when putting this into a two's complement calculator. The two's complement of this number in binary is 0101 0011 0100 1101 1011 0110 1101 1100 which equals -1397602012 in decimal how, how is this a negative integer when the most significant bit is zero? Zero means positive sign.