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Bromocresol green is used as a pH indicator and as a tracking dye for DNA agarose gel electrophoresis. Please read up on the properties and
Bromocresol green is used as a pH indicator and as a tracking dye for DNA agarose gel electrophoresis. Please read up on the properties and use of Bromocresol green by using the following link: Table 11-3 lists as transition range 3.8-5.4. The acid color is given as yellow and the base color as blue. A. Please draw the acid dissociation reaction of the indicator in aqueous solution. What role does the pka value play in this context? B. What color do you expect to observe for bromocresol green indicator at the following pH value (a): 3.5 (b) 5.0 (c) 6.0. C. Please refer to Harris (10th) Figure 11-9 "Calculated titration curve for the reaction of 100 mLM base (pKb=5) with 0.050MHCl."; Is Bromocresol green a useful indicator for this titration? Bromocresol green is used as a pH indicator and as a tracking dye for DNA agarose gel electrophoresis. Please read up on the properties and use of Bromocresol green by using the following link: Table 11-3 lists as transition range 3.8-5.4. The acid color is given as yellow and the base color as blue. A. Please draw the acid dissociation reaction of the indicator in aqueous solution. What role does the pka value play in this context? B. What color do you expect to observe for bromocresol green indicator at the following pH value (a): 3.5 (b) 5.0 (c) 6.0. C. Please refer to Harris (10th) Figure 11-9 "Calculated titration curve for the reaction of 100 mLM base (pKb=5) with 0.050MHCl."; Is Bromocresol green a useful indicator for this titration
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