By examining the genomes of healthy people and comparing them to the genomes of people with a genetic disorder, you identify a gene that appears to be mutated in the people with the disorder. How would you go about producing the protein encoded by the gene in bacteria? The gene contains several introns interspersed among the exons. 

Combine the techniques you would use. 

What would you do first? 

What would this allow you to do next? 

What other methods would you use?

 

Polymerase Chain reaction cDNA synthesis Restriction endonuclease DNA cloning in situ hybridization quantitative PCR (qPCR) Microarray RNA sequencing Crispr-CAS9 RNAI GWAS Amplification of DNA Make DNA copy of an RNA Cut/clone DNA Amplify DNA segments in bacteria Make proteins in bacteria What cells are expressing mRNA When and how much is a gene expressed What genes are being expressed (all) What genes are being expressed (all) Knock out gene function Knock out gene function Associate genes with phenotype Comparison to DNA databases Sequence comparison, identification I DNA replication Nucleic Acid Hybrdization DNA replication RNA virus replication Bacterial defense Bacterial plasmids Nucleic Acid Hybrdization RNA virus replication DNA replication RNA virus replication Nucleic Acid Hybrdization RNA virus replication DNA replication Bacterial defense Bacterial defense Pedigrees/sequence/computation Conservation of DNA