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( c ) A mechanobiological experiment is used to examine the biochemical response of cells to a fluid shear stress in 2 D monolayer culture.

(c) A mechanobiological experiment is used to examine the biochemical response of cells to
a fluid shear stress in 2D monolayer culture. For this experiment a parallel plate flow
chamber is used, and the internal dimensions of the chamber are 0.45mm(height) and
55mm(width).
i. Assuming that the viscosity of perfusion media is 8.910-4Pa.s, and the density
of media is 997kgm-3, estimate the required flow velocity of the media if a shear
stress of 2Pa is to be imparted onto the cell monolayer.
ii. Determine whether these flow conditions result in a laminar flow regime.
iii. Calculate the entry length for plane Poiseuille flow to develop.
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