The aim of this experiment is to determine the partition coefficient of succinic acid in system of two immiscible liquids: 1-butanol - water. The measurement will be performed at 4 different concentrations of succinic acid and different volume ratios of partitioned mixture (1-butanol - water). CH-COOH CH-COOH Reaction for succinic acid and sodium hydroxide 1 16. MATERIALS 2024 = 80 - Succinic acid (aqueous solution c-20g/dm) 0.05 M NaOH (500 mls) 1-butanol, Phenolphthalein PROCEDURE: Funnel No. 1 2 3- 2NaOH 3 4 1. Prepare mixtures of butanol, water and succinic acid in separation funnels as outlined in the table below. 3. 2. Gently shake the separating funnels with prepared solutions for 20 min. Allow mixtures to stand and separate into two clear layers: lower layer is water (Wat), and upper layer is butanol (Org). Pour carefully the water phase into a beaker using the funnel's valve. layers). Table: Composition of Mixtures. 1 4. For each mixture titrate 10 ml aliquots of the water phase against NaOH using phenolphthalein as indicator. Rem; All titrations must be repeated. 5. Repeat step 4 for the butanol phase (note; discard the interface between both. 4 (29)//250ml -50 m go5x0-25=0.0125 mols. &=0.0125 x 40- (0.5 used 3g Succinic acid (ml) 40 20 30 20 CH2-COONa CH2-COONa water Wat I Water (ml) 30 . 20 + 2 HO 1-butanol (ml) 30 30 X 30 x 30 NaOH Titrat at 5.5ml 3.5 2.5 m 3.5 CALCULATIONS 1. Calculate the concentration of succinic acid in the water and organic phase, cWat, cOrg, respectively, for each composition of the partition mixture 2. Calculate the average partition coefficient k of succinic acid at each composition of thepartition mixture. K=[Succinic]wa/[ Succinic]org layer water layer 1 organic layer 1 water layer 4 organic layer 4 titre using (NaOH) at 5.5 ml 3.5 ml 2.5 ml 3.5 ml