Calculate the transformation efficiencies for each group. Show me one example calculation and organize your data in a table. Is there a trend in transformation efficiencies? Why would transformation efficiencies differ between APV+I, V+I, APV, and positive pUC19 control? How would you expect them to differ between groups?

Volume plated= 50,100,200 ul Total transformation volumes=310ul pg DNA used= 101.7ng = 101700pg

I've been using this formula, and here is my work for one. I'm I doing this correctly?

APV+1, 50ul, W:15

number of colonies/Volume plated (ul) * total transformation/pg DNA used * 10^6pg/ug = 914/1ug DNA

Group APV+I V+I APV pUC19 10 L N/A N/A N/A B: 6 W: 0 50 L B: 0 W: 15 B: 39 W: 10 B: 2 W:0 N/A 100 L B:1 W:34 B: 79 W: 21 B: 4 W: 0 B: 55 W:0 200 L B:8 W:53 B: 74 W:20 B: 8 W: 0 N/A