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Carbon is introduced to the cycle from acetyl coenzyme A (AcSCOA), which citrate synthase reacts with oxaloacetate to provide citrate. Citrate has a prochiral centre,

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Carbon is introduced to the cycle from acetyl coenzyme A (AcSCOA), which citrate synthase reacts with oxaloacetate to provide citrate. Citrate has a prochiral centre, indicated with * below. H20 HSCOA ,,, SCOA citrate synthase AcSCOA oxaloacetate citrate a. The two new carbons (from AcSCOA) are introduced as the pro-s group in citrate. Is this the group to the left or right as drawn above? [2] b. In order for AcsCoA to react with oxaloacetate, the enzyme must first deprotonate the AcSCOA. Given this information, which substrate (AcSCOA or oxaloacetate) is reacting as the nucleophile? The electrophile? Explain your reasoning. C. Explain why the protons of the methyl group in AcSCOA are acidic. [3] [3]

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