Question
The graph below shows an overlay of the van Deemter plots for three HPLC columns with different stationary phase packing particle sizes. With reference
The graph below shows an overlay of the van Deemter plots for three HPLC columns with different stationary phase packing particle sizes. With reference to the van Deepter equation, explain the shape of the plots, why the shapes of the plots are different, and also explain what useful information can be gained by studying van Deemter curves. 30 25 Column 1 20 15 10 Column 2 Column 3 6. Flow rate (mL/min) H.E.T.P (um)
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