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Chondrocytes are cultured in vitro in order to seed the cartilage scaffold represented below: a. Calculate N, the total number of chondrocytes necessary to populate
Chondrocytes are cultured in vitro in order to seed the cartilage scaffold represented below: a. Calculate N, the total number of chondrocytes necessary to populate the scaffold at a final density of 1107cells/cm3. b. Knowing that chondrocytes undergo a tenfold expansion in culture, what is the initial number of cells (N0) required to obtain N ? c. Considering that the relationship between N and N0 is: N/N0=ekt where k=(ln2)/tD and the approximate doubling time of cells is tD=36 hours, calculate how long it would take (t) to grow the total number of cells needed to populate the scaffold (N), given your starting population (N0)
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