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Complete hydrolysis of a polypeptide M yields: Ala, Arg, Gly, 2 Lys, Met, Phe, Pro, 2 Ser, Tyr, Val Deduce the amino acid sequence of

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Complete hydrolysis of a polypeptide M yields: Ala, Arg, Gly, 2 Lys, Met, Phe, Pro, 2 Ser, Tyr, Val Deduce the amino acid sequence of polypeptide M based on the following data: i. Treatment with Edman's reagent gives PTH-Val ii. Treatment with carboxypeptidase A releases Ala iii. Treatment with cyanogen bromide yields the following two peptides: 1. Ala, 2 Lys, Phe, Pro, Ser, Tyr 2. Arg, Gly, Met, Ser, Val - 2 - SS iv. Treatment with trypsin yields the following three peptides: 1. Gly, Lys, Met, Tyr 2. Ala, Lys, Phe, Pro, Ser 3. Arg, Ser, Val Treatment with chymotrypsin yields the following three peptides: 1. 2 Lys, Phe, Pro 2. Arg, Gly, Met, Ser, Tyr, Val 3. Ala, Ser V

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