1. What determines if a bacterial cell is Grampositive or Gramnegative 

2 Why was the Gram iodine added to the Gram staining procedure 

3 Why is a counterstain (safranin) added to the Gram staining procedure 

4 What are the advantages of performing a Gram stain vs a simple stain for visualizing bacteria 

5 How would each of the following impact the Gram stain results 

a Over decolorization 

b Under decolorization 

c Reversal of crystal violet and safranin 

d Failure to use Grams iodine 

6 Give an example of a situation in a research lab or medical setting in which Gram staining would be utilized and discuss.