Consider a case study where a 71.4 kg woman was admitted to your hospital the previous morning and has been commenced on a schedule of one 25 mg tablet four times a day. The morning pathology services have collected 5 mL of blood for you to analyse. You can assume that:

i. the patient has a blood volume of 70 mL per kg body weight

ii. there was 100% bio availability of the drug iii. there has been no elimination of imipramine or its metabolites Calculate the amount of imipramine and its metabolites in this sample and then discuss a method that would allow you to analyse for these materials at these concentrations. Detail the key features and differences expected for imipramine, desmethy limipramine and 2-hydroxy imipramine with your method?