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Consider the peaks for pentafluorobenzene and benzene in the gas chromatogram shown here. The elution time for unretained solute is 1.06min. The open tubular column
Consider the peaks for pentafluorobenzene and benzene in the gas chromatogram shown here. The elution time for unretained solute is 1.06min. The open tubular column is 30.0m in length and 0.530mm in diameter, with a layer of stationary phase 3.0m thick on the inner wall. (a) Find the adjusted retention times and retention factors for both compounds. (b) Find the relative retention, . (c) Measuring w1/2 on the chromatogram, find the number of plates, N1 and N2, and the plate height for these two compounds. (d) Measuring the width, w, at the baseline on the chromatogram, find the number of plates for these two compounds. (e) Use your answer to (d) to find the resolution between the two peaks. (f) Using the number of plates N=N1N2, with values from (d), calculate what the resolution should be and compare your answer with the measured resolution in (e)
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