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Construct a figure of Lineweaver-Burk plot. Determine the K M and V max of the enzyme-substrate complex from this figure. Please make sure that your

Construct a figure of Lineweaver-Burk plot. Determine the KM and Vmax of the enzyme-substrate complex from this figure. Please make sure that your figure has all the necessary components such as axis title, axis scale, axis unit etc.

concentration (in mM) of substrate (NPP):

0.005mM

0.01mM

0.025mM

0.5mM

0.075mM

0.1mM

0.125mM

p-nitrophenol (PNP) :

0.00021mMmin1

0.00023mMmin1

0.00029mMmin-1

0.00043mMmin 1

0.00069mMmin-1

0.00028mMmin 1

0.00055mMmin 1

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