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Construct two hypothetical van Deemter plots and write down the related equations for gas and liquid chromatographic columns. Is there any significant difference between these
Construct two hypothetical van Deemter plots and write down the related equations for gas and liquid chromatographic columns. Is there any significant difference between these two plots? Why? Explain the meanings of A B and C terms. Then use the equations to explain how and why the particle size in a packed HPLC column affects the column efficiency and the pressure required for pumping mobile phase through the column.
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