Determination of molecular weight of a protein You want to determine the molecular weights of unknown proteins by use of SDS-PAGE. The following table shows the migration distance of each of the standard proteins (markers). Name of protein molecular weight (dalton) migration distance (cm) MBP-B-galctodidase rabbit phosphorylase b bovine serum albumin aldolase B-Lactoglobulin A 175.000 97,400 66,500 47,500 25,000 1.5 1.9 2.2 2.5 3.0 The migration distances of two unknown proteins were as follows: Unknown Protein A: 2.1 cm Unknown Protein B: 2.7 cm Determine the molecular weights of these two unknown proteins. Your lab report should include the following: (1) Complete the data table shown above. (The last column needed for the standard curve should be filled in.) (2) Construct the standard curve using Excel (scatter plot needed), determine the trendline and obtain the equation for the line. (3) Calculate the molecular weights of Unknown Protein A and Unknown Protein B. You need to show the calculations. Determination of molecular weight of a protein You want to determine the molecular weights of unknown proteins by use of SDS-PAGE. The following table shows the migration distance of each of the standard proteins (markers). Name of protein molecular weight (dalton) migration distance (cm) MBP-B-galctodidase rabbit phosphorylase b bovine serum albumin aldolase B-Lactoglobulin A 175.000 97,400 66,500 47,500 25,000 1.5 1.9 2.2 2.5 3.0 The migration distances of two unknown proteins were as follows: Unknown Protein A: 2.1 cm Unknown Protein B: 2.7 cm Determine the molecular weights of these two unknown proteins. Your lab report should include the following: (1) Complete the data table shown above. (The last column needed for the standard curve should be filled in.) (2) Construct the standard curve using Excel (scatter plot needed), determine the trendline and obtain the equation for the line. (3) Calculate the molecular weights of Unknown Protein A and Unknown Protein B. You need to show the calculations