Diafiltration - need of water and loss of product Diafiltration can be used to purify your product (if you do not know what diafiltration means, please, check from the course materials). Your hemicellulose extract contains 60 g/L hemicelluloses and 10 g/L monomeric sugars. You need to remove 95% of monomeric sugars from the extract. Your membrane retains 90% of the hemicelluloses and 20% of the monomeric sugars. How much water is needed in diafiltration to remove 95% of monomeric sugars. What is the loss of hemicelluloses when purity is achieved? Cend = Cpe-(1-R)DV VOIA DV = VE 8 = 1- e-(1-R)DV Cend CF R Concentration in the end of the fractionation filtration, g/L Concentration in the feed tank, g/L Retention, % Volume of the solvent in diafiltration, L Volume of the feed, L Loss of compound, g VDIA VF 8