DNA concentration. The nucleotide bases of DNA and RNA are aromatic and absorb ultraviolet light. The concentration of DNA or RNA can be determined by monitoring absorbance at 260 nm. Calibration data for a 15-base single-stranded DNA (4568 g/mol) in a 1-cm cell are shown in the table. m a Absorbance DNA (ug/mL) 2.5 0.080 5.0 0.149 10.0 0.307 15.0 0.442 20.0 0.620 25.0 0.739 Analysis of a DNA sample in triplicate with a hand-held photometer yielded a mean concentration of 8.257 ug/mL and standard deviation of 0.019 ug/mL. A commercial spectrometer gave 8.386 + 0.159 ug/mL for n = 3. Use the F-test to determine whether the precisions of the hand-held photometer and the commercial spectrometer are statistically equivalent at the 95% confidence level. Report the calculated F value and the F value from the table. Fcalculated = Ftable = Enter numeric value Are the precisions statistically equivalent at the 95% confidence level? no O yes Use an appropriate t-test to determine whether the concentrations are statistically equivalent at the 95% confidence level. Report the calculated t value and the t value from the table. t calculated = ttable = Are the concentrations statistically equivalent at the 95% confidence level? yes no