Drug release calculations Drug Release Homework: You have been asked to study plot the \% cumulative release from a microsphere formulation and asked to define the release mechanism using the following information. Drug- Celecoxib Assay Method- UV visible spectroscopy. Wavelength-252nm Formulation-Glutaraldehyde cross-linked gelatin microspheres Size range 100200m. Theoretical loading- 20% Loading efficiency is 95% Amount formulation used for the release- 250mg Dissolution Media PBS pH 7.4+0.1\% Tween 80 Amount of dissolution media- 100mL Each time-5mL media was taken out and replenished at each measurement Following table provide you the information on Time and Absorbance of drug released Calculate and answer the following questions. 1. Calculate the amount of drug present in 250mg formulation. 2. Plot % cumulative Release. 3. Plot Higuchi plot and discuss the drug diffusion mechanism 4. Calculate the left over drug in the formulation after 10 hours