1. During the last part of the lab, we try to calculate the kinetic parameters Km and Vmax using two graphic methods, designated Michaelis-Menten and Lineweaver-Burk. What we do is measure nitrophenol appearance for up to 150 seconds using different substrate concentrations. The following table shows data recorded during this experiment.

[Substrate]	Absorbance	[Nitrophenol]	V0	1/ [Substrate]	1/V0
0	0.11			undefined
50	0.166
100	0.237
250	0.304
500	0.317
750	0.345
1000	0.340

1. Calculate nitrophenol produced using your standard curve
2. Calculate the initial velocity (V₀) by dividing the nitrophenol concentration obtained by 150 s (or the time during which we measured the reaction)
3. Calculate 1/[Substrate] and 1/V₀
4. Plot the Michaelis-Menten and Lineweaver-Burk Plots.
5. From each of the graphs, obtain Km and Vmax.

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