During the procedure of cloning of a gene of interest (GOI) in the pUC19 plasmid, both vector and fragment to be inserted were treated with alkaline phosphatase following restriction enzyme digestion. After ligation and transformation steps, no colonies were found on the agar plate. Give a brief description of how dephosphorylation is used in DNA cloning and explain why this result may have occurred. Propose a modification to the approach that would allow to obtain colonies carrying the desired recombinant plasmid.