• Enzyme Denaturation with Temperature Calculate the value of the denaturation constant kd for a given enzyme, using the equation provided in below. Where A _d = 3x10 ⁴⁷ and E _d = 60 kcal/mole (use a temperature of 45°C). Then, prepare a plot of per cent enzyme maximal activity vs temperature, using an E _a of 15 kcal/mole, and an enzyme concentration E _o of unity. Note that the y-axis of this plot is a per cent value, so the value of A in the Arrhenius equation does not need to be known in this case. On the denaturation side of the curve, use 5 minutes as the constant exposure time.