EXERCISE 2 THE EFFECT OF PH ON BACTERIAL GROWTH Data Sheet Name ... ... Date Lab Section ......... I Was Present And Performed This Exercise (Initials)..... Observations And Interpretations Record Your Absorbance Values Or Numeric Values For Each Organism At Each PH. Organism PHP PHPH PH? 279 Classification Uninoculated |PH3/PHS/PHz Pha Control #2 #3

 

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Name Lab Section EXERCISE THE EFFECT OF PH ON BACTERIAL GROWTH Data Sheet Date........ I was present and performed this exercise (initials)... Observations and Interpretations Record your absorbance values or numeric values for each organism at each pH. Organism Uninoculated PH PH PH PH Control PH3 PHS PH PHa # 1 0.02 0.21 0.63 0.18 #2 0.02 0.11 0.32 0.51 #3 0.15 0.53 0.41 0.02 #4 0.05 0.36 0.66 0.22 If using a spectrophotometers, enter the absorbance. Enter visual readings as 0, 1, 2, or 3. Questions Classification Circle the pH optimum for each organism. Place brackets around the range. Is there any overlap between species? Account for the inability of organisms to grow outside their pH ranges. Why, for instance, are alkaliphiles able to survive at high pHs when neutrophiles cannot? Too high or too low of pH impacts enzymes and oteins and they can't grow. In higher pH alkalili designed to function whereas as low enzymes are not designed to function zymes are designed +