Experimental Determination of AG for ATP Hydrolysis A direct measurement of the standard free-energy change associated...
Fantastic news! We've Found the answer you've been seeking!
Question:
Transcribed Image Text:
Experimental Determination of AG" for ATP Hydrolysis A direct measurement of the standard free-energy change associated with the hydrolysis of ATP is technically demanding because the minute amount of ATP remaining at equilibrium is difficult to measure accurately. The value of AG" can be calculated indirectly, however, from the equilibrium constants of two other enzymatic reactions having less favorable equilibrium constants: Glucose 6-phosphate + HO glucose + P Keq = 270 ATP + glucoseADP + glucose 6-phosphate Keq = 890 Using this information for equilibrium constants determined at 25 C, calculate the standard free energy of hydrolysis of ATP. Experimental Determination of AG" for ATP Hydrolysis A direct measurement of the standard free-energy change associated with the hydrolysis of ATP is technically demanding because the minute amount of ATP remaining at equilibrium is difficult to measure accurately. The value of AG" can be calculated indirectly, however, from the equilibrium constants of two other enzymatic reactions having less favorable equilibrium constants: Glucose 6-phosphate + HO glucose + P Keq = 270 ATP + glucoseADP + glucose 6-phosphate Keq = 890 Using this information for equilibrium constants determined at 25 C, calculate the standard free energy of hydrolysis of ATP.
Expert Answer:
Related Book For
Posted Date:
Students also viewed these biology questions
-
Citrate synthase catalyzes the reaction The standard free energy change for the reaction is -31.5 kJ mol-1. (a) Calculate the equilibrium constant for this reaction at 37°C. (b) Would you expect...
-
The standard free energy of formation of solid glycine is -369 kJ/mol, whereas that of solid glycylglycine is -488 kJ/mol. What is G for the condensation of glycine to form glycylglycine?
-
The standard free energy of activation of a reaction A is 90 kJ mol-1 (21.5 kcal mol-1) at 298 K. Reaction B is one million times faster than reaction A at the same temperature. The products of each...
-
Write a static method lg() that takes an int argument n and returns the largest integer not larger than the base-2 logarithm of n. Do not use the Math library.
-
How does an electronic transition resemble a vibrational transition? How do they differ?
-
What role does information play in strategic planning in healthcare?
-
Use the same facts as in problem 31 except that Brandlin Company purchases parts from a foreign sup plier on December 1, 2009, with payment of 20,000 korunas to be made on March 1, 2010. On December...
-
Laya Corporation produced 200,000 watches that it sold for $16 each during 2012. The company determined that fixed manufacturing cost per unit was $7 per watch. The company reported an $800,000 gross...
-
confused X Company, a merchandiser, started business on June 1. The following were June transactions: 1. Received $83,000 from a group of investors. 2. Bought $8,340 of merchandise, $3,409 for cash...
-
The financial statements for Harridges Ltd are given below for the two years ended 30 June 2009 and 2010. Harridges Limited operates a department store in the centre of a small town. Dividends were...
-
The polynomial function f(x)=x^(3)-3x^(2)+kx+24 has roots at 4,-3, and 2 . What is the value of k ? 2 answers
-
1. A T-shaped beam with an overhang is supported and loaded as shown in Fig. 1. Draw shear force diagram and calculate (a) the shear stress at a point D, 2 m from support A and 25 mm from the top of...
-
Question 1: Write Specific Case to brief is Flying Fish Bikes, Inc. v. Giant Bicycle, Inc., 181 F.Supp.3d 957? Grading Rubric for Case Brief Written Case Brief 3 (Exceeds Identification - Heading...
-
A local gym is looking in to purchasing more exercise equipment and runs a survey to find out the preference in exercise equipment amongst their members. They categorize the members based on how...
-
A fountain with an opening of radius 0.015 m shoots a stream of water vertically from ground level at 6.0 m/s. The density of water is 1000 kg/m. (a) Calculate the volume rate of flow of water. (b)...
-
35 h/2 21 3 3 2t 3. A thin-walled beam has the cross-section shown in the figure. If the beam is subjected to a bending moment Mx in the plane of the web 23 calculate the direct stress at the points...
-
Home Lawn and Garden Company, Inc. had these transactions during the current period. March 2 2 Issued 4 0 , 0 0 0 shares of $ 3 stated value common stock for cash of $ 1 6 0 , 0 0 0 . May 1 1 Issued...
-
Suppose that you could invest in the following projects but have only $30,000 to invest. How would you make your decision and which projects would you invest in? Project Cost $ 8,000 11,000 9,000...
-
What is the pressure in a 50.0-L gas cylinder that contains 3.03 kg of oxygen, O2, at 23oC?
-
If the n quantum number of an atomic orbital is 4, what are the possible values of l? If the l quantum number is 3, what are the possible values of ml?
-
A second-order reaction has a rate constant of 8.7 104/(Ms) at 30oC. At 40oC, the rate constant is 1.8 103/(Ms). What are the activation energy and frequency factor for this reaction? Predict the...
-
Cisco Systems, Inc. (CSCO), manufactures and sells networking and communications equipment for transporting data, voice, and video and provides services related to that equipment. Its products...
-
By any stretch of the imagination, Cisco Systems (CSCO) has been a strong growth company. A darling of the Internet boom of the late 1990s, it was one of the few technol- ogy companies tied to the...
-
E14.14. Valuation Grid and Reverse Engineering for Home Depot, Inc. (Medium) 2. Using the information in Exercise 14.13, calculate the implied growth rate in residual operating income that is...
Study smarter with the SolutionInn App