Explain why initial velocity measurements for enzyme assays are usually more accurate than end-point determinations. Explain why it is not enough to just plot absorbance changes vs. substrate concentrations i.e. why you had to do all those calculations! Compare any published kinetic values of yeast ADH with your results and conclusions. Comment on the accuracy (or otherwise) of your experiments. In the human body, alcohol is mainly metabolized by ADH in liver cytoplasm and through the microsomal ethanol oxidizing system (MEOS) of which the main enzyme is CYP2E1. The KM of ADH for ethanol is 0.04 mM whilst that of CYP2E1 is 11 mM. Which system is likely to be operating more effectively in a person caught drink-driving with a blood alcohol concentration (BAC) of 20 mM? Explain your answer.