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Exponential growth in a batch process. You're running a CHO process in a batch reactor. Biomass ( x ) is formed from substrate ( S

Exponential growth in a batch process. You're running a CHO process in a batch reactor. Biomass
(x) is formed from substrate (S). Using the data tabulated below, determine the specific growth rate
() using the exponential growth model. Reminder: Be sure to account for the lag phase.
Mass balances and rates in a batch fermenter. You are running a 5L batch fermentation process
that produces biomass (x) and consumes substrate (S). You have the following plots: (1)ln(xtx0)
vs time and (2)(S(t)-So) vs (x(t)-xo) :
a. Calculate the specific growth rate () and the doubling time (d).
b. Calculate the specific substrate consumption rate (qs).
c. Given: MWs=26gC-mol and MWx=24gC-mol. How many grams of S is consumed per
grams of x produced?
d. Now you're going to scale up the process from 5L to 1000L. Assume you run the 5L and 1000L
processes at the same conditions (initial concentrations, temperature, pH, etc.). Compare the
values of the variables/parameters listed below between the 5L process and the 1000L
process. Which will be higher in the 1000L, and which will be the same at both scales?
d
q5
Substrate concentration at t=2d
Mass of substrate at t=2d
e. Assume that your 3 day, 5L batch process produces 5g of Product P. If the yearly demand for
P is 100kg, can your 1000L process meet the demand? Assume that it takes 6 hours to clean
and sterilize the fermenter between batches.
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