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TABLET Values of kinetic constants and ratios of rate constants for the B-galactosidases ND indicates that values were not determined in this study. The intercepts are from apprent versus (Rent - appka)/(A) plots where A is methanol or 2-mercaptoethanol. The errors given for Rent and Km are standard errors which were obtained by the least squares method described by Cornish-Bowden (1976) assuming simple weighting. For the intercepts of the lines with methanol and mercaptoethanol, standard errors of the least squares fits to the plots are also given. The R values (regression coefficients) for each of the plots with acceptor added were all greater than 0.95. ken K. ky ks Methanol Mercaptoethanol intercept intercept $ $! ONPG 750 + 30 0.12 + 0.02 2,100 1,200 ND ND REH4 285 + 15 0.16 0.03 2,320-2,990 315-325 640 + 2 92 +0.7 (R=0.95) (R = 0.98) MM s! s! Wild type PNPG Wild type 90 + 5 120 + 10 0.039 + 0.003 0.16 + 0.01 90 190-195 1,200 315-325 REH4 ND 1903 (R=0.96) ND 66 + 0.6 (R = 1.00) Lactose Wild type 60 + 10 REH4 315 +40 . From Tenu et al, 1971. 1.4 +0.3 2.7 +0.2 1,200 60 >1,500 ND ND ND ND 315-325 (a) Recall that for neural networks with sigmoid activation functions of the form = 1 0(z) = 1 + exp(-2) the value of neuron k at layer / is computed as *-of-* ) = = + Whisiv! - 1 iLayerl-1