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Figure 1 below shows the strategies for purifying supercoiled plasmid DNA. oo Fermentation Centrifugation Cell lysis Filtration Clarification and concentration Single or dual mode Chromatography
Figure 1 below shows the strategies for purifying supercoiled plasmid DNA. oo Fermentation Centrifugation Cell lysis Filtration Clarification and concentration Single or dual mode Chromatography Figure 1: Downstream process to obtain pure supercoiled plasmid DNA from E.coli fermentation As can be seen single or dual mode chromatography is required in purifying the supercoiled plasmid DNA. Suggest TWO chromatography that can be used in getting pure supercoiled plasmid DNA. Justify your
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