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Find the structures, molecular masses, and physical properties (especially melting points) of the lipids trimyristin, triolein, and cholesterol. How much of your 1.0MCaCl2 will be
Find the structures, molecular masses, and physical properties (especially melting points) of the lipids trimyristin, triolein, and cholesterol. How much of your 1.0MCaCl2 will be added to 5.0mL of Tris buffer for a final concentration of 10. mM of the calcium ion cofactor of the lipase enzyme? It is important not to underload (can't detect compound) or overload (streaking and no separation of mixtures) the TLC plate. Say I weighed 2.4mg of a waxy solid like trimyristin, how much chloroform should I add so that spotting with a 5.0L microcap loads 20.g in that dried spot on the plate. (Remember, can't use 10. L microcap because too drippy with chloroform.)
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