From a series of batch runs with a constant enzyme concentration, the following initial rate data were obtained as a function of initial substrate. Evaluate the Michaelis-Menten kinetic parameters by employing the Langmuir plot, the Lineweaver-burk plot, the Eadie Hofstee plot, and a nonlinear regression technique. in evaluating the Kinetic parameters do not include data points which deviate systematically from Michaelis-Menten model and explain the reason for the deviation?

substrate concentration (mmol/l) initial reaction rate (mmol/l min)

1 0.20

2 0.22

3 0.30

5 0.45

7 0.41

10 0.50

15 0.40

20 0.33