In the production of a biotherapeutic drug, a suspension containing media A and RO water are added to another suspension of media B in a bioreactor B-01 as shown in the schematic below. I Media suspension A Volume = 300L Media density 1.1 kg/L = Cp = 4.4 kJ/kg.K nitial temp = 20C arget temp = 35C Q = 5 kW Q = 5 kW Mixing vessel V01 xv. XV-01 RO storage vessel S-01 XV-02 Bioreactor B-01 RO water Volume = 200L Water density = 1 kg/L Cp = 4.2 kJ/kg.K Initial temp = 20C Target temp = 30C Media suspension B Volume = 200L Density 1.35 kg/L Cp = 4.7 kJ/kg.K Initial temp = 25C Target temp = 38C Q = 6 kW The process is as follows: (1) Mixing vessel V-01 is heated from 20C to 35C. The jacket on this vessel provides 5 kW of heating power to the vessel. (2) At the same time the RO water storage tank, S-01, starts heating from 20C to 30C. The jacket on this vessel provides 6 KW of heating power. BE224 - Bioprocess Engineering Principles Repeat Examinations 2021/2022 (3) Once both vessels have reached their respective target set points, valves XV-01 and XV-02 automatically open and transfer contents from both vessels to bioreactor B-01. Flow rates through valves XV-01 and XV-01 are throttled to 10 L/min and 8 L/min respectively. After exactly 5 minutes of transfer both valves close. (4) Simultaneously to Step (3), the contents of the bioreactor B-01 starts heating from 25C to 38C. Once the feed valves close the vessel continues to heat up to target temperature. The bioreactor jacket provides 5 kW of heating power to the vessel. [5 Marks] (a) State all of the assumptions that you are making about the system. State the boundary and initial conditions for each step of the process as you work through your answers below. (b) [20 Marks] Approximately how long do Steps (1) & (2) take, i.e. when will the contents of both vessels be ready for transfer to the bioreactor? (c) [25 Marks] Once transfer to the bioreactor has started, how long does it take for the bioreactor to reach the target temperature of 38C.