From physical chemistry viewpoint comment if enthalpy of activation H, kJ/mol; entropy of activation S, J/mol K, and; free energy of activation (Gibbs), G could be used to predict the passage between reversibility and irreversibility of enzyme deactivation (thermal)? i) Write your assumptions. ii) What other parameters you should measure? iii) Can enzymes recover after thermal deactivation? iv) Why the 3-D conformation of enzymes are important?