Question
Hello, I was able to get pretty far in my homework, but I need help figuring out this last question. Question: The pKa for any
Hello, I was able to get pretty far in my homework, but I need help figuring out this last question.
Question: The pKa for any weak acid can be determined from the Henderson Hasselbalch Equation pH = pKa + log [A- ] [HA]. Notice that this equation is like that for a straight line; y = mx + b. Using data from our table, we can take advantage of this fact to find an estimate for the pKa of p-nitrophenol.
For example, if we make the following substitutions: y = pH m = slope of the line = 1.0 (each increase in pH represents a directly proportional increase in log [A- ]/[HA] x = log [A- ]/[HA] b = pKa Then we can estimate the pKa for p-nitrophenol from a graph of pH versus log [A- ]/[HA] Prepare a second graph (Figure 2) of pH (y-axis) versus log [A- ]/[HA] (x-axis) using the values from Table 1. Use the scatter plot option in Microsoft Excel or similar and produce a line of best fit (regression) through the data points.
pH | Absorbance | [A-] (mM) | [HA] (mM) | log [A-]/[HA] |
9.0 | 0.516 | 0.00004mM | 0mM | N/A |
8.0 | 0.409 | 4.09 X 10^-4mM | 1.82 X 10^-4 | 1.63 |
7.5 | 0.308 | 3.08 X 10^-4 | 1.71 X 10^-4 | -0.52 |
7.0 | 0.256 | 2.56 X 10^-4 | 7.11 X 10^-5 | -0.6 |
6.5 | 0.108 | 1.08 X 10^-4 | 1.57 X 10^-5 | -1.3 |
6.0 | 0.007 | 7.0 X 10^-7 | 1.9 X 10^-8 | -3.5 |
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