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QUESTION 1 What are three of the most widely planted GM crops? soybeans canola O cotton Opapaya. O All of the above QUESTION 2 A transgene is a gene that has been transferred naturally, or by any of a number of genetic engineering techniques, from one organism to another. What are some example of genes that have been introduced into plants? O genes that encode herbicide and insect resistance O genes that increase plant yields genes that encode drought and frost tolerance O All of the above QUESTION 5 The gene we will test for during this lab codes for a protein that allows crops to grow better in the presence of the herbicide Roundup True O False QUESTION 6 Order the following steps required to determine if a food sample is genetically modified Determine the presence of the transgene Isolate DNA from food samples Run an agarose gel electrophoresis Amplify the GMO transgene QUESTION 3 What are two important transgenes (transferred genes) that have been widely introduced into crop plants? (choose all that apply) Bt gene Bacillus thuringiensis genome Glyphosate-resistance gene Roundup Ready gene QUESTION 4 In the present lab, you will test for the presence of which transgene? Tubulin gene Bt gene Glyphosate-resistance gene Roundup Ready gene Bacillus thuringiensis gonomo QUESTION 7 To determine the presence of a genetically modified organism, which specific DNA sequence will we amplify? Tubulin gene Bacillus thuringiensis gene O Glyphosate-resistance gene O 359 promoter QUESTION 8 Why is it important to also amplify the tubulin gene from our samples? O Tubulin amplification is a positive control To show that the extracted DNA is of good quality To allow the transgene to bind tubulin To confirm that the protein is found in all plants QUESTION 9 All good scientific experiments should have controls. A negative control isn't expected to produce the desired results. A positive control should produce the desired results. When testing for the presence of the transgene, which of the following is correct? The positive control will be a tissue sample from Roundup Ready soy plants O The positve control will be samples from wild type plants The negative controls will be Roundup Ready soy plants There will be no controls in this experiment QUESTION 10 DNA extraction requires different chemicals Match each reagent or stop with its function during the extraction process (p 8-9 of the handout explains this in detail) Edwards' buffer A Contains detergent that dissolves ipids of the cell membrane Boiling the sample B. denatures proteins, including DNA-digesting enzymes Isopropanol c Degrades RNA molecules TE (Tris EDTA) buffer D. precipitates nucleic acids RNase E Dissolve the DNA