Hexokinase catalyzes the reaction between glucose and ATP: HO-P-OH LOH H H OH Hexokinase H H OH HO H HO OH OH H OH Glucose ATP OH ADP Glucose 6-phosphate Glucose-6-phosphate is a common intermediate in the cell. In comparison to glucose, it is more reactive and it has a good leaving group (phosphate) for group transfer reactionsucleophilic substitutions. Adenosine triphosphate (ATP): NH2 HO- OH OH OH OH OH 1. Identify the nucleophile and the electrophile of the reaction. Identify if they are weak or strong. (2 pts) 2. Write the mechanism of the 1st step of the reaction with all arrows. Which group will leave, so that the P will have 5 bonds again? (2 pts) 3. What second step will have to occur so that all products will be neutral? Write the mechanism for the 2nd step. (2 pts) What steps does the enzyme have to go through to catalyze this reaction? Remember, you are starting with an empty active site. (1 pt) 5. How does the active site catalyze the reaction, i.e. lower the activation energy? (2 pts) 6. What class of enzyme is hexokinase? In organic chemistry, what is the mechanism called? (1 pt) 4